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. 2021 Aug 2;40(18):e107100. doi: 10.15252/embj.2020107100

Figure EV3. Deletion of de novo DNA methyltransferases does not influence long‐term maintenance of adult hippocampal NSPCs in vivo .

Figure EV3

  1. Validation of genomic deletion of Dnmt3a (3A) and Dnmt3b (3B) in Gfp‐positive cells by polymerase chain reaction. Arrows indicate expected sizes for wildtype Dnmt3a and Dnmt3b amplicons.
  2. No difference in the percentage of NSPCs and astrocytes among Gfp‐positive cells was detected between WT (red; n = 3 mice) and KO mice (violet; n = 4 mice). Corresponding experimental scheme is depicted in Fig 4E. Depicted are data points for every animal with genotype means ± SEM.
  3. Representative fluorescent image for detection of NSPCs and astrocytes. Depicted astrocyte is Gfp‐negative. Scale bar: 50 µm.
  4. Experimental outline for results presented in E‐G and in Fig  5D and E.
  5. No difference was found in the total numbers of NSPCs in the subgranular zone (SGZ) between WT and KO mice 3 months after tamoxifen administration (n = 13 mice, WT; n = 15 mice, KO). Depicted are data points for every animal with genotype means ± SEM (P‐value from unpaired t‐test).
  6. Representative fluorescent image for analysis of Sox2‐positive NSPCs. Scale bar: 100 µm.
  7. No difference in the total numbers of proliferating cells in the SGZ between WT and KO mice 3 months after recombination (n = 7 mice per genotype). Depicted are data points for every animal with genotype means ± SEM (P‐value from unpaired t‐test).