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. 2021 Aug 2;40(18):e107100. doi: 10.15252/embj.2020107100

Figure 7. Blocking de novo DNA methylation during adult hippocampal neurogenesis impairs hippocampus‐dependent learning and memory.

Figure 7

  1. Mice with adult NSPC‐specific deletion of Dnmt3a and Dnmt3b (KO) and wildtype littermates (WT) were tested in the Morris water maze 3 months after administration of tamoxifen (n = 13 mice, WT; n = 23 mice, KO).
  2. Protocol for the Morris water maze test.
  3. Average distance animals swam per day to find the hidden platform.
  4. Average time animals needed to reach the platform per day. Note that KO mice showed increased path length and latency on days 4 and 5 compared to WT mice.
  5. KO mice spent on day 5 more time in the quadrant in which the goal was located during the acquisition phase compared to WT mice.
  6. Heatmaps depicting the percentage of individual search strategies used by WT and KO mice per trial (left). Color scheme for the classification into spatial and non‐spatial search strategies (right).
  7. Odds ratio of strategy use in KO compared to WT mice separated by acquisition and reversal phase (P‐values were determined by linear regression).

Data information: *P < 0.05; **P < 0.01; ***P < 0.001. Panels (C–E) show means ± SEM. Unpaired Mann–Whitney test was used to determine significance in (C–E).