FIGURE 2.

Inflammatory transcriptome of aged skeletal muscle macrophages is blunted after 4 days of reloading. (a) Gene set enrichment analysis (GSEA) of top regulated pathways from single cell sequencing transcripts in pro‐inflammatory macrophage clusters from day 4 of reloading after disuse. (b) Bar graphs of significantly different select inflammatory transcripts in young and old pro‐inflammatory macrophages from day 4 of reloading after disuse. (c) Individual GSEA analysis demonstrating enriched inflammatory signaling in young pro‐inflammatory macrophages from day 4 of reloading after disuse atrophy. NES denotes normalized enrichment score. A positive NES denotes enriched in young compared to old. Wilcoxon rank‐sum test was used to rank genes and determine significance for GSEA and boxplot analysis. Statistical significance is p < 0.05