TABLE 1.
Cell culture, passage, and gene expressed | PCR product (CpG cytosinesa)
|
Mean no. of methylated sites per 47 total CpG sitesb | |||||||
---|---|---|---|---|---|---|---|---|---|
A (1–15)
|
B (13–47)
|
||||||||
No. of PCR clones sequenced | No. of methylated CpG sites/clone
|
No. of PCR clones sequenced | No. of methylated CpG sites/clone
|
||||||
Meanc | Median | Range | Mean | Median | Range | ||||
HMEC9 | |||||||||
3 | 10 | 0.1 (0.0, 0.3) | 0 | 0–1 | 10 | 0.5 (0.0, 1.1) | 0 | 0–2 | 0.6 |
8 | 9 | 0.2 (0.0, 0.5) | 0 | 0–1 | 9 | 0.2 (0.0, 0.5) | 0 | 0–1 | 0.4 |
16, E6 | 10 | 2.9 (1.5, 4.3) | 2 | 1–8 | 11 | 9.1 (7.0, 11.2) | 9 | 5–16 | 11.8 |
29, E6 | 7 | 2.9 (1.7, 4.1) | 3 | 1–5 | 10 | 13.6 (12.2, 15.0) | 14 | 10–16 | 16.2 |
42, E6 | 10 | 4.6 (2.5, 6.7) | 3.5 | 1–11 | 12 | 19.2 (15.4, 23.0) | 20 | 9–28 | 23.0 |
32, E6/E7 | 10 | 0.0 (0.0, 0.0) | 0 | 0 | 10 | 0.2 (0.0, 0.5) | 0 | 0–1 | 0.2 |
HMEC6 | |||||||||
3 | 10 | 0.0 (0.0, 0.0) | 0 | 0 | 11 | 0.4 (0.0, 0.8) | 0 | 0–2 | 0.4 |
12, E6 | 10 | 2.0 (0.7, 3.3) | 1 | 0–5 | 12 | 9.3 (7.2, 11.4) | 8.5 | 6–17 | 10.9 |
25, E6 | 10 | 2.1 (1.1, 3.1) | 2.5 | 0–4 | 12 | 19.2 (16.4, 22.0) | 19 | 9–25 | 20.6 |
HMEC4 | |||||||||
5 | 10 | 0.9 (0.0, 2.1) | 0 | 0–5 | 10 | 0.7 (0.0, 1.5) | 0 | 0–3 | 1.5 |
12 | 10 | 1.5 (0.4, 2.6) | 1.5 | 0–4 | 12 | 8.3 (4.7, 11.9) | 8.5 | 0–17 | 9.5 |
18 | 9 | 2.9 (2.3, 3.5) | 3 | 2–4 | 12 | 5.4 (3.2, 7.6) | 6 | 1–11 | 7.7 |
H249 | 4 | 0.0 (0.0, 0.0) | 0 | 0 | 4 | 0.0 (0.0, 0.0) | 0 | 0 | 0.0 |
H1618 | 3 | 14.7 (13.3, 15.0) | 15 | 14–15 | 4 | 35.0 (35.0, 35.0) | 35 | 35 | 46.9 |
Calculated by adding the methylation frequency per clone at each of the 47 sites (the methylation frequencies at CpG sites 13 to 15 were calculated with data from both PCR product A and B clones).
Ninety-five percent confidence intervals of the means are shown in parentheses.