TABLE 2.
Kinetic parameters of RBDWT and mutated RBDs calculated by BLI
| Analyte | KD [M] | kon [M−1s−1] | koff [s−1] |
|---|---|---|---|
| RBDWT | 20.5 × 10−9 | 1.34 × 105 | 2.91 × 10−3 |
| RBDTRIP | 10.3 × 10−9 | 1.69 × 105 | 1.75 × 10−3 |
| RBDN501Y | 6.2 × 10−9 | 1.69 × 105 | 1.85 × 10−3 |
| RBDE484K | 19.7 × 10−9 | 1.64 × 105 | 3.24 × 10−3 |
| RBDK417N | ND | ND | ND |
Abbreviations: ND, not determined; RBDE484K, Receptor‐Binding Domain E484K mutation; RBDK417N, Receptor‐Binding Domain K417N mutation; RBDN501Y, Receptor‐Binding Domain N501Y mutation; RBDTRIP, Receptor‐Binding Domain N501Y, E484K, K417N mutations; RBDWT, Receptor‐Binding Domain wild type.