FIG. 3.
Overexpression of Bcl-2 or Bcl-XL has no effect on TG-induced changes in mitochondrial and cytosolic free-calcium levels at 3 h. JT/Neo, JT/Bcl-2, and JT/Bcl-XL cells were treated with TG (50 nM) for 3 h, loaded with indo-1/AM and rhod-2, and analyzed for mitochondrial (A) and cytosolic free (B) calcium levels by confocal imaging microscopy (see Materials and Methods for details). The data are the means and standard errors. Significant differences (P < 0.05) among groups were determined by analysis of variance with multiple comparisons by the Student-Neuman Keul test and are indicated by different letters. Histograms denoted by a common letter are not significantly different in group comparisons. Means among groups denoted by dissimilar letters are statistically significant.