FIG. 5.
Inhibition of TG- or CPA-induced apoptosis by chelating intracellular calcium or overexpressing Bcl-2 and Bcl-XL. (A) Time course of TG-induced apoptosis in JT/Neo, JT/Bcl-2, and JT/Bcl-XL cells. Cells were treated with TG (50 nM) for 4, 6, 18, 24, 36, and 48 h. (B and C) Chelation of intracellular calcium by BAPTA-AM blocks TG- or CPA-induced apoptosis. Cells were pretreated with BAPTA-AM (10 μM) for 45 min, washed, reseeded and treated with TG (10, 50, or 100 nM), or CPA (0.1, 1, or 10 μM) for 36 h. (D and E) Overexpression of Bcl-2 or Bcl-XL blocks TG- or CPA-induced apoptosis. Cells were treated with TG (10, 50, or 100 nM) or CPA (0.1, 1, or 10 μM) for 36 h. A nucleosome ELISA was used to measure apoptosis.