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. 2021 Sep 8;24(5):786. doi: 10.3892/mmr.2021.12426

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Copyright: © Sun et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Identification of potential binding miRNAs of circFLNA in glioblastoma. (A) Subcellular localization of circFLNA was determined using RT-qPCR in LN229 cells. (B) Expression levels of miR-199-3p, miR-296-5p and miR-515-5p in LN229 cells transfected with pcDNA3.1-circFLNA were analyzed using RT-qPCR. Expression levels of miR-199-3p were downregulated following transfection with pcDNA3.1-circFLNA. (C) RT-qPCR was used to determine that the expression levels of miR-199-3p were upregulated following the transfection with si-circFLNA. Data are presented as the mean ± SD of three independent experiments. **P<0.01, ***P<0.001. miR, microRNA; NC, negative control; si, small interfering RNA; circFLNA, circular RNA filamin A; RT-qPCR, reverse transcription-quantitative PCR.