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. 2021 Sep 9;24(5):789. doi: 10.3892/mmr.2021.12429

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — INHBA negatively regulates Hippo signaling by downregulating Merlin protein expression levels in lung cancer cells. (A) Western blotting revealed that only Merlin protein expression levels were decreased in H1299 cells transfected with the INHBA overexpression plasmid. (B) Western blotting revealed the efficacy of Merlin overexpression plasmids in H1299 cells. (C) Rescue experiments showed that overexpression of Merlin reversed the effect of INHBA on activating YAP in H1299 cells. (D) Reverse transcription-quantitative PCR indicated no change in NF2 (encoding Merlin) mRNA expression levels after H1299 cells were transiently transfected with the INHBA plasmid. (E) Immunoprecipitation analysis revealed that INHBA could bind to Merlin. *P<0.05, **P<0.01, ***P<0.001. INHBA, inhibin βA; YAP, yes-associated protein; WWC1, WW and C2 domain-containing 1; FRMD6, FERM domain-containing 6; NF2, neurofibromin 2.