Figure 7.

Changes in SNU-1 cell apoptosis after inhibiting autophagy with 3-MA. (A) Changes in the apoptosis of SNU-1 cells after Nar (10 µg/ml), 3-MA and Nar (10 µg/ml) plus 3-MA treatment were analyzed by flow cytometry analysis. (B) Changes in autophagy-related proteins and caspase 3 of SNU-1 cells after Nar (10 µg/ml), 3-MA and Nar (10 µg/ml) plus 3-MA treatment were analyzed by western blot analysis. GAPDH expression was used to normalize the data. *P<0.05, **P<0.01, ***P<0.001 vs. Nar (10 µg/ml) group; ^#P<0.05 vs. Nar (10 µg/ml) plus 3-MA group. Nar, naringin; LC3B, microtubule-associated protein 1 light chain 3 β; p-, phosphorylated.