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. 2021 Jul 30;22(2):e00133-21. doi: 10.1128/jmbe.00133-21

TABLE 1.

Lab manual Table of Contents illustrating the topics

Lab topic Learning objectives
Evolution: Geological Time, Primate and Human Evolution, and Molecular Evolution
  • Identify major geological and evolutionary events

  • Create a scaled timeline of major evolutionary events and indicate the approximate date of each

  • Calculate the proportion of earth’s history for which various groups of organisms have existed

  • List derived characteristics of primates and humans

  • Distinguish between primitive and advanced characteristics in primate facial and skull bones

  • Analyze evolutionary relationships using molecular (DNA) evidence

Evidence of Evolution
  • Describe evidence of evolution that is based on microevolution and population genetics

  • Define and use the terminology of population genetics correctly

  • Use the equations of the Hardy-Weinberg equilibrium to calculate allele and genotype frequencies

  • Graph allele frequencies using Microsoft Excel and identify changes in allele frequencies

  • Draw graphs and explain three types of selection

Taxonomy and phylogenetics
  • Explain how the following evidence is used for phylogenetic reconstruction: the fossil record, DNA, and biogeography.

  • Describe specific examples of phylogenetic reconstruction, such as the relationship of humans to other primates.

  • Build and analyze a phylogenetic tree, identifying patterns of shared ancestry.

  • Differentiate between the allopatric and sympatric modes of speciation.

Bacteria
  • Identify and define common bacterial shapes and features, including: cocci, bacilli, spirilli, pili, capsule, spore, fimbriae, flagella, plasmid, Gram-positive cell wall, Gram-negative cell wall.

  • Describe various bacterial metabolic processes, including: photosynthesis, chemosynthesis, methanogenesis, nitrogen fixation.

  • Identify and describe at least three vital roles that bacteria play in their ecosystems, such as primary production, decomposition, nitrogen fixation, and disease.

Protista
  • Define the term “protist” and explain why this is not a monophyletic group.

  • Identify representatives from each supergroup Excavata, “SAR” clade, Archaeplastida, and Unikonta

  • Draw a phylogenetic tree for the eukaryotes and explain why the eukaryote supergroups form a polytomy.

  • Indicate the position of plants, animals, and fungi on the eukaryote tree, and identify the group of protists most closely related to each.

  • Give examples of protist species from each eukaryote supergroup.

  • Give two examples of the significant impact of specific protists on their ecosystems.

Fungi
  • Describe fungal classification into phyla, and provide a phylogeny of Kingdom Fungi.

  • In a sentence or two, describe the characteristics of the three largest phyla in the Kingdom Fungi (Zygomycota, Ascomycota, Basidiomycota).

  • Using images, explain the life cycle of typical multicellular fungi.

  • Give three examples of how humans benefit from specific uses of fungi

Plants I: Seedless plants
  • Distinguish members of Kingdom Plantae from their nearest relatives (charophyte algae)

  • Draw a basic phylogeny for Kingdom Plantae

  • Diagram and explain the life cycle of plants (alteration of generations)

  • Differentiate the characteristics of nonvascular plants vs seedless vascular plants

  • Using images, explain the life cycles of moss (a nonvascular plant) and ferns (a seedless vascular plant)

Plants II: Seed plants
  • Describe the alternation of generations life cycle in plants

  • List characteristics of gymnosperms and angiosperms

  • Identify reproductive structures in gymnosperms and angiosperms

  • Summarize differences between monocots and eudicots

  • Label the reproductive and non-reproductive structures of a flower

Animals I: Invertebrates
  • Distinguish members of the Kingdom Animalia from their closest living relative (Choanoflagellates and Fungi).

  • Explain the basic body plan of members in the Kingdom Animalia.

  • Identify members of the Phyla Porifera, Cnidaria, Platyhelminthes, Rotifera, Annelida, Mollusca, Nematoda, Arthropoda, Echinodermata.

  • Compare two types of invertebrate life cycles.

  • Compare the structure and function of invertebrates.

Animals II: Vertebrates
  • List characteristics found in the Subphylum Vertebrata of Kingdom Animalia

  • List characteristics of each of the major tetrapod groups: amphibians, reptiles, birds, mammals; and provide examples of each

  • Identify homologous structures in vertebrates, and explain the functions of each structure

  • Identify representatives from the eight vertebrate clades, Agnatha, Chondrichthyes, Osteichthyes (comprised of Actinopterygii and Sarcopterygii), Amphibia, Reptilia, Aves, and Mammalia

  • Identify and list 11 organ systems in vertebrate animals, their main organs, and provide the major function(s) of each (integumentary, skeletal, muscular, nervous, endocrine, digestive, respiratory, cardiovascular, lymphatic/immune, urinary, & reproductive)

  • Compare the life cycles of amphibians and mammals

  • Identify structures in dissected specimens of representative vertebrates (frog and fetal pig)

Ecology I: Biomes, Population Growth and Predator-Prey Dynamics
  • Identify the characteristics of Earth’s major terrestrial biomes and describe the impacts humans have had on these biomes.

  • Explain the relationships between climate (temp and precipitation) and terrestrial biome type.

  • Apply the concepts of biotic potential and environmental resistance to human population growth.

  • Explain the difference between exponential and logistic growth and define carrying capacity.

  • Identify major events that have affected human population growth, and explain how they have increased carrying capacity.

  • Explain the dynamics in population size in a real-life predator-prey relationship.

  • Explain the difference between density-dependent and density-independent factors that affect population growth.

  • Interpret real-life predator-prey population data as depicted in a graph.

Ecology II: Community and Ecosystem Dynamics
  • Explore the concept of an ecological niche and the difference between a fundamental niche and a realized niche using an example of two barnacle species competing for the same resource.

  • Using the same two competing barnacle species, demonstrate how limiting factors (predation and desiccation tolerance) interact to result in competitive exclusion, resource partitioning and realized niches.

  • Investigate the concept of trophic cascades, and explain how a keystone species can indirectly affect the biodiversity and nutrient cycling of an entire ecosystem.