FIG. 4.

RK3E lines transformed by mutated β-cat form colonies in agar. Colony formation in soft agar was assessed for parental RK3E cells, a polyclonal RK3E line transformed by mutant K-Ras, and four representative β-cat-transformed lines. A total of 10⁴ cells of each line were plated in 0.3% agar medium over agar underlayers. After 3 weeks, the dishes were stained with methylene blue and the colonies were photographed.