FIG. 6.

c-myc expression is not uniformly activated in stable RK3E lines transformed by mutated β-cat. Northern blot studies of c-myc, candidate c-Myc-regulated genes (lactate dehydrogenase A [LDH-A] and ornithine decarboxylase [ODC]), and a loading control (glyceraldehyde-3-phosphate dehydrogenase [GAPDH]) were carried out on total RNA from parental RK3E cells, a polyclonal RK3E line transformed by activated K-Ras (RK3E/Kras), and 10 different RK3E lines transformed by mutated β-cat. All β-cat-transformed lines form colonies in soft agar, and several of the lines were tested and found to grow in nude mice (see the text for details).