Figure 6.

Modulation of immune response by extracellular vesicles in Dsg2^mt/mt mice. (A) Representative cytokine array with quantification of relative intensity. (B, C) Cytokine levels in ventricular samples (n = 4 in each group). (D) Representative western blots (top) and quantified protein levels (bottom) of key immune regulators and cytokines of samples from wild type, vehicle-, extracellular vesicle-treated Dsg2^mt/mt mice ventricles. (E) Representative western blots (left) and quantified protein levels (right) of macrophage lineage marker CD68 in wild type, vehicle-, extracellular vesicle-treated Dsg2^mt/mt mouse ventricles. (F) Immunohistochemical staining for CD68 and α-sarcomeric actinin (ASA) in wild type, vehicle-, extracellular vesicle-treated Dsg2^mt/mt mice. Scale bars: 100 μm. Data are mean ± SD. P-values: one-way ANOVA with the Tukey’s multiple-comparisons test.