Figure 8.

Antagomir-4488 blunts extracellular vesicle therapeutic effects in Dsg2^mt/mt mice. (A) Experimental protocol. (B–E) Echo of wild type, extracellular vesicle + antagomir-scramble- (Si-scr), and extracellular vesicle + antagomir-4488-(Si-4488)-treated Dsg2^mt/mt mice (n = 4 per group). (F) Incidence of pacing-induced ventricular tachycardia. (G) Representative Masson’s trichrome–stained micrographs from wild type, extracellular vesicle + antagomir-scramble- (Si-scr), and extracellular vesicle + antagomir-4488-(Si-4488)-treated Dsg2^mt/mt mice. Scale bars for ventricle overview: 1 mm; left ventricle: 300 μm. (H) Quantification of fibrotic area (n = 4 per group). Data are mean ± SD; P-values: one-way ANOVA with the Tukey’s multiple-comparisons test.