Table 2.
Percent rhythmicity, period and power of activity rhythms in wildtype, Pdf0 and perS flies under T-cycle entrainment.
| T-Cycle | n | n Rhythmic (%) | Period (SEM), h | Power (SEM) | n | Phase of Evening Peak (SEM) ZT, h |
|
|---|---|---|---|---|---|---|---|
| wildtype 8 h |
T22 | 31 | 31 (100) | 22.00 (0.00) | 101.01 (9.34) | 31 | 8.83 (0.21) |
| T24 | 31 | 31 (100) | 24.04 (0.02) | 117.05 (9.15) | 31 | 8.30 (0.04) | |
| T26 | 30 | 30 (100) | 26.00 (0.00) | 165.67 (13.05) | 30 | 7.24 (0.11) | |
| T28 | 30 | 20 (93.33) | 28.02 (0.02) | 136.13 (12.75) | 27 | 6.66 (0.13) | |
|
Pdf0 8 h |
T22 | 31 | 31 (100) | 21.96 (0.02) | 116.42 (9.43) | 31 | 7.24 (0.12) |
| T24 | 29 | 29 (100) | 23.98 (0.02) | 169.25 (10.71) | 29 | 5.71 (0.18) | |
| T26 | 31 | 31 (100) | 25.95 (0.02) | 139.01 (10.00) | 28 | 4.44 (0.20) | |
| T28 | 31 | 31 (100) | 28.02 (0.03) | 163.36 (14.63) | 31 | 2.56 (0.16) | |
| wildtype 12 h |
T22 | 31 | 29 (93.55) | 22.00 (0.00) | 129.55 (12.07) | 29 | 12.02 (0.12) |
| T24 | 28 | 25 (89.29) | 23.99 (0.01) | 157.32 (17.34) | 24 | 11.78 (0.12) | |
| T26 | 29 | 29 (100) | 26.00 (0.00) | 164.11 (13.06) | 28 | 11.29 (0.08) | |
| T28 | 31 | 29 (93.55) | 28.01 (0.01) | 147.64 (16.55) | 28 | 10.61 (0.11) | |
| T30 | 29 | 29 (100) | 30.10 (0.05) | 132.06 (14.39) | 27 | 9.04 (0.15) | |
|
Pdf0 12 h |
T22 | 32 | 30 (93.75) | 21.99 (0.01) | 128.28 (12.32) | 29 | 10.49 (0.15) |
| T24 | 30 | 29 (96.67) | 23.93 (0.04) | 146.51 (11.5) | 28 | 8.35 (0.20) | |
| T26 | 32 | 27 (84.38) | 25.94 (0.04) | 150.14 (14.15) | 27 | 6.71 (0.25) | |
| T28 | 26 | 24 (92.31) | 27.91 (0.05) | 115.11 (12.47) | 23 | 5.18 (0.24) | |
| T30 | 23 | 23 (100) | 29.93 (0.05) | 186.09 (17.37) | 23 | 2.38 (0.25) | |
|
perS 12 h |
T22 | 31 | 28 (90.32) | 21.99 (0.01) | 160.04 (14.47) | 28 | 9.93 (0.12) |
| T24 | 28 | 27 (96.43) | 24.00 (0.01) | 174.72 (13.95) | 28 | 8.48 (0.11) | |
| T26 | 30 | 28 (93.33) | 26.00 (0.03) | 122.61 (14.31) | 25 | 7.60 (0.12) | |
| T28 | 30 | 26 (86.67) | 28.00 (0.02) | 121.04 (14.93) | 26 | 5.71 (0.11) | |
| T30 | 30 | 27 (90.00) | 30.03 (0.03) | 114.59 (15.76) | 27 | 3.43 (0.14) | |
| wildtype 16 h |
T22 | 31 | 31 (100) | 22.00 (0.00) | 254.39 (15.38) | 31 | 15.31 (0.07) |
| T24 | 31 | 31 (100) | 24.00 (0.00) | 209.15 (13.68) | 31 | 14.81 (0.12) | |
| T26 | 30 | 30 (100) | 26.00 (0.00) | 225.76 (15.9) | 30 | 14.35 (0.13) | |
| T28 | 27 | 27 (100) | 28.00 (0.00) | 224.56 (18.13) | 27 | 13.10 (0.20) | |
| T30 | 30 | 29 (96.67) | 30.01 (0.02) | 136.52 (14.72) | 26 | 12.69 (0.21) | |
| T32 | 30 | 30 (100) | 32.00 (0.02) | 259.54 (20.98) | 28 | 10.58 (0.16) | |
|
Pdf0 16 h |
T22 | 31 | 28 (90.32) | 21.94 (0.03) | 107.94 (13.84) | 28 | 13.06 (0.20) |
| T24 | 29 | 27 (93.10) | 23.94 (0.03) | 188.49 (15.08) | 27 | 11.48 (0.23) | |
| T26 | 31 | 30 (96.77) | 25.98 (0.03) | 119.65 (12.27) | 30 | 9.98 (0.14) | |
| T28 | 29 | 29 (100) | 27.95 (0.04) | 121.93 (9.19) | 29 | 8.02 (0.23) | |
| T30 | 31 | 31 (100) | 29.97 (0.05) | 168.79 (13.74) | 31 | 5.81 (0.21) | |
| T32 | 29 | 29 (100) | 31.95 (0.05) | 162.58 (13.5) | 27 | 3.25 (0.18) |
Abbreviations: SEM = Standard Error of Mean; ZT = zeitgeber Time. Six cycles of activity data from individual fly under T-cycle entrainment was analysed using Chi-square periodogram method (5% level of significance) to test rhythmicity and estimate period. Data from rhythmic flies was further analysed to estimate phase of E activity peak.