Table 6.
Contribution of the 24 h or 32 h periodicity, their statistical significance and timings of peak phases of nuclear PER in clock neurons.
| PR | p-Value | Peak (SE) | PR | p-Value | Peak (SE) | |
|---|---|---|---|---|---|---|
| ZT, h | ZT, h | |||||
| T24 | T32 | |||||
| Wildtype | ||||||
| sLNv | 68.81 | <.001 | 3.29 (0.34) | 67.67 | <.001 | 31.23 (0.41) |
| lLNv | 60.18 | <.001 | 3.07 (0.41) | 74.00 | <.001 | 28.99 (0.35) |
| 5th LNv | 67.77 | <.001 | 3.34 (0.35) | 50.66 | <.001 | 27.83 (0.58) |
| LNd | 80.71 | <.001 | 3.35 (0.25) | 77.94 | <.001 | 28.22 (0.31) |
| DN1 | 84.70 | <.001 | 2.37 (0.21) | 85.68 | <.001 | 28.35 (0.23) |
| DN3 | 70.91 | <.001 | 3.40 (0.33) | 52.84 | <.001 | 26.27 (0.56) |
| Pdf0 | ||||||
| sLNv | 78.06 | <.001 | 2.97 (0.27) | 66.61 | <.001 | 28.87 (0.42) |
| lLNv | 73.79 | <.001 | 2.15 (0.30) | 70.37 | <.001 | 29.13 (0.38) |
| 5th LNv | 72.08 | <.001 | 2.13 (0.31) | 56.26 | <.001 | 24.44 (0.52) |
| LNd | 84.28 | <.001 | 2.13 (0.22) | 67.03 | <.001 | 25.16 (0.41) |
| DN1 | 82.34 | <.001 | 1.90 (0.23) | 79.73 | <.001 | 28.10 (0.29) |
| DN3 | 64.53 | <.001 | 2.78 (0.38) | 21.81 | <.001 | 26.49 (1.12) |
Abbreviations: PER = PERIOD protein; PR = percentage rhythm; ZT = zeitgeber Time; sLNvs = small lateral ventral neurons; lLNvs = large lateral ventral neurons; LNds = lateral dorsal neurons; DN1 = dorsal neurons 1; DN3 = dorsal neurons 3. The single-component COSINOR based method was used to analyse time series of PER staining intensities in individual sub-groups of clock neurons to detect rhythmicity and estimate rhythm parameters. The statistic—PR estimates the strength of periodic component being tested in the analysis. The COSINOR being a regression-based method; PR is analogues to an R2 value (coefficient of determination) which represents percentage of overall variation explained by the fitted model. The p-values are from F-tests for the statistical significance of the period being tested.