Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 9;22(5):773. doi: 10.3892/ol.2021.13034

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 3. — TMCC1-AS1 promotes proliferation in liver cancer cells. (A) RT-qPCR was used to analyze the expression levels of TMCC1-AS1 in HepG2 and SNU-182 cells transfected with si-TMCC1-AS1#1 or si-TMCC1-AS1#2. (B) Cell viability was determined in the aforementioned transfected HepG2 and SNU-182 cells using a CCK-8 assay. (C) Cell proliferation was assessed using a colony formation assay in HepG2 and SNU-182 cells transfected with si-TMCC1-AS1#1. (D) RT-qPCR was used to analyze the expression levels of TMCC1-AS1 in HepG2 and SNU-182 cells transfected with pcDNA3.1-TMCC1-AS1 or pcDNA3.1. (E) Cell viability was determined in the aforementioned transfected HepG2 and SNU-182 cells using a CCK-8 assay. (F) Cell proliferation was assessed using a colony formation assay in HepG2 and SNU-182 cells transfected with pcDNA3.1-TMCC1-AS1. Data are presented as the mean ± SD of three independent experiments in vitro. **P<0.01, ***P<0.001 vs. si-NC or pcDNA3.1. Scale bar, 50 µm. CCK-8, Cell Counting Kit-8; NC, negative control; OD, optical density; RT-qPCR, reverse transcription-quantitative PCR; si, small interfering RNA; TMCC1-AS1, long non-coding RNA transmembrane and coiled-coil domain family 1 antisense RNA 1.