TABLE 1.
Antibody or staining reagents used in this study
| Antibody /cell staining reagent | Clone, Isotype | Label‐fluorescent |
|---|---|---|
| Mouse anti‐bovine CD4 (Bio‐Rad) | CC8, IgG2a | FITC (Bio‐Rad) or Alexa 647 (Bio‐Rad) |
| Mouse anti‐bovine CD8 (Bio‐Rad) | CC63, IgG2a | no‐label or APC‐Cy7, lightning‐link (abcam) |
| Mouse anti‐bovine TCR1‐N24 delta chain (Washington State University) | GB21A, IgG2b | no‐label or APC‐Cy7, lightning‐link (abcam) |
| Mouse anti‐bovine CD14 (Bio‐Rad) | CC‐G33, IgG1 | no‐label or APC‐Cy7, lightning‐link (abcam) |
| Mouse anti‐bovine CD21 (Bio‐Rad) | CC21, IgG1 | no‐label or APC‐Cy7, lightning‐link (abcam) |
| Mouse anti‐bovine IFN‐γ (Bio‐Rad) | CC302, IgG1 | FITC (Bio‐Rad) |
| Mouse anti‐bovine IL−4 (Bio‐Rad) | CC303, IgG2a | FITC (Bio‐Rad) |
| Mouse anti‐human IL−17A (Mabtech) | MT504, IgG1 | FITC (Mabtech) |
| Mouse anti‐bovine CD45RO (Bio‐Rad) | IL‐A116, IgG3 | PerCP/Cy5·5, lightning‐link (abcam) |
| Rat anti‐human CCR7 (BD Pharmingen) | 3D12, IgG2aκ | Alexa 647(BD Pharmingen) |
| Mouse IgG1 isotype control (Bio‐Rad) | aCat:MCA928F, IgG1 | FITC (Bio‐Rad) |
| Mouse IgG2a isotype control (Bio‐Rad) | OX−34, IgG2a | FITC (Bio‐Rad) |
| Mouse IgG3 isotype control (Bio‐Rad) | aCat:MCA5920, IgG3 | PerCP/Cy5·5, lightning‐link (abcam) |
| Rat IgG2aκ isotype control (BD Pharmingen) | R35‐95, IgG2aκ | Alexa 647(BD Pharmingen) |
| CellTrace CFSE cell proliferation kit (Invitrogen) | ||
| 7‐Aminoactinomycin D (7‐AAD, Invitrogen) | ||
| Fixable Viability Dye eFluor 780 (Invitrogen) |
a
Catalogue number of mAb.