FIGURE 5.

PTCy reduces histological damage to the liver in humanized NSG mice. (a–c) NSG mice were injected (i.p.) with 20 × 10⁶ hPBMCs and subsequently injected (i.p.) with 33 mg kg⁻¹ PTCy or saline on days 3 and 4 post‐hPBMC injection. (a–f) At end‐point, samples of (a, d) liver, (b, e) small intestines and (c, f) skin tissue from saline‐mice (a–c) and PTCy‐mice (d–f) were sectioned (5 µm) and assessed by haematoxylin and eosin staining. (g–l) Liver tissue from PTCy‐mice and saline‐mice was sectioned (5 µm) and assessed for infiltrating (g, h) hCD4⁺ (red arrows) and (j, k) hCD8⁺ T cells (yellow arrows) using immunohistochemistry by staining with anti‐human CD4 and anti‐human CD8, respectively. (i, l) Data represent the counts of (i) hCD4⁺ and (l) hCD8⁺ T cells around fields of vessel and interstitial tissue normalized to mm² of tissue sections. Bars represent 50 μm. Images represent tissues from four mice per group. GVHD, graft‐versus‐host disease, hPBMCs, human peripheral blood mononuclear cells, i.p, intraperitoneal, NSG, NOD‐scid‐IL2Rγ^null, PTCy, post‐transplant cyclophosphamide