FIGURE 5.
TLR2 dimers differentially regulate IL‐10 and IL‐12 in the presence of PKC‐α and PKC‐β inhibitors. Effect of PKC‐α and PKC‐β inhibition on TLR2‐induced IL‐10 and IL‐12 in macrophages. (A) BALB/c‐derived peritoneal macrophages were seeded at density of 106 cells per well and allowed for 48‐h resting. The cells were treated with indicated doses of specific PKC isoform inhibitor in serum‐free media for 2 h. Cells were further treated with TLR2 ligands 50 ng/ml P3C, 5 μg/ml PGN and 50 ng/ml FSL and incubated for another 48 h. Cell‐free supernatants were collected and estimated for IL‐10 and IL‐12 production by ELISA. The experiments were repeated twice, and data from one of the experiments are shown. The data represent mean ± SEM. The amount of IL‐10 and IL‐12 production was compared with untreated cells, and values P < 0·05 (*), P < 0·01 (**) and P < 0·001 (***) were considered to be statistically significant