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Primers for qRT-PCR amplification
| Purpose gene | Primer direction | Primer sequences (5′-3′) | Product length (bp) |
|---|---|---|---|
| IL-2 | Forward | TTCATCTCGAGCTCTACACACCAA | 200 |
| Reverse | GCATTCACTTCCGGTGTGATTTA | ||
| IL-10 | Forward | GAGCTGAGGGTGAAGTTTGAGGA | 85 |
| Reverse | GTTCAGAGCTGAGCAGTTGGATGT | ||
| IFN-γ | Forward | AGCATTTGAACTGAGCCATCACC | 181 |
| Reverse | CCGTCAGCTACATCTGAATGACTTG | ||
| TGF-β | Forward | ACCTCGACACCGACTACTGCTTC | 126 |
| Reverse | CCATATAACCTTTGGGTTCGTGGA | ||
| CTLA-4 | Forward | TCTGCAAGATGGAGCGGATG | 174 |
| Reverse | CGACAATGGCTGAGATGATGATG |
