Fig. 2.

NRXN1α^+/- deletion significantly impairs voltage-gated Na⁺ currents. Representative traces of voltage clamp recording from A control (n = 45 cells from 29 coverslips) and from B NRXN1α^+/- (n = 27 cells on 17 coverslips) iPSC-derived neurons, showing fast inward Na⁺ currents followed by slow outward K⁺ currents, in response to different voltage step applied. C Average Na⁺ and K⁺ currents from both controls (black) and NRXN1α^+/- (red) neurons, showing significant increases of the inward Na⁺ in a voltage-dependent manner in the NRXN1α^+/- neurons. D The data from panel C were normalized to maximal Na⁺ (G/Gmax) E, and also normalized to Na⁺ conductance (I/Imax) and plotted against voltage steps, with significant increase at − 30 mV (** p < 0.002) and no significant change at − 20 mV (p = 0.23). Data were presented as mean ± SEM. Statistical significance (** p < 0.01) were evaluated using Man Whitney U test or repeated measure ANOVA