Fig. 4.

Overview of differential expressed genes in NRXN1a^+/- ASD neurons and qRT-PCR validation. A An overview of the connected protein–protein interaction network of NRXN1a^+/- targets, showing 269 down-regulated (in green) and 156 upregulated genes (in purple). All DEGs are FDR < 0.05, TPM > 2, either < 50% reduction (in green) or > twofold increase (in purple). B Validation of the RNASeq by qRT-PCR in five control (02VC1, 3VC2, 3VCX1, 4C3, 4CX1) and three ASD (ND1C1, ND2C11, ND4-1C1) lines, which showed reduction of GRIN3B (0.33x) and the upregulation of GRM1 (1.86x, p = 0.18), SHANK1 (2.28x, *p = 0.05), CACNA1A (1.93x, *p = 0.05) and SLC17A6 (3.47x, **p = 0.01) respectively