FIG. 7.

Expression of Dfz2 is reduced in zygotic N⁻ embryos, while expression of en and wg are unaffected. (A to D) In situ hybridization of Canton S and nd³ embryos corroborates results from Northern blotting that Dfz2 is overexpressed in nd³ embryos but en is not (E to H, G′, H′) Dfz2 expression is lost in N^264-47/Y embryos but not in comparable stages of Canton S or Dl^X/Dl^X embryos. (I to L) wg expression is not lost in N^264-47/Y. (M to P) en expression is similar in Canton S and N^264-47/Y embryos. CS, Canton S; nd³, nd³; N, N^264-47/Y; Dl, Dl^X/Dl^X embryos; genes used as probes are indicated below the appropriate sets of embryos. Anterior is to the left of each embryo. N^264-47/Y and Dl^X/Dl^X embryos were identified by lack of β-galactosidase staining associated with the FM7 or TM6 balancer chromosomes (see Materials and Methods). Embryos A to D were processed simultaneously, and so were embryos E to P.