FIG. 8.
Ax embryos overproduce Dfz2 and sgg RNA. (A to L) Ax embryos overproduce Dfz2 RNA (A, C, E, G, I, and K) but not en RNA (B, D, F, H, J, and L). (M to R) Ax embryos overproduce sgg RNA (O to Q) but not Canton S (M and R) and N264-47/Y embryos (N). Due to low-level of expression in a general pattern, reduced sgg expression (as in panel N) and weak sgg overexpression (as in panel O) are more obvious in pools of embryos than in individual embryos. CS, Canton S; Ax9, Ax9B/Y; spl Ax59, spl Ax59d/Y; Ax59, Ax59d/Y; N, N264-47/Y. Anterior is to the left of each embryo. Homozygous Ax or N embryos were identified by the lack of β-galactosidase staining associated with the FM7 balancer chromosomes (see Materials and Methods). Embryos A to H and M to P were processed simultaneously, and so were embryos I to L and Q to R.