Extended Data Fig. 2 |. Control groups do not differ in their defensive responses to looming threat.

Refers to Figs. 2–4. a–h, Comparison of different control mice reveals no significant differences across any of the behaviours performed in response to the looming threat: tail rattling (a, e), running (b, f), freezing (c, g), or hiding (d, h). Controls include: mice with no treatments; mice with AAV-XFP and CNO; mice with CAV-Cre, AAV-DIO-XFP and CNO; mice with CAV-Cre and AAV-DIO-hM3Dq but without CNO; and mice with AAV-XFP, optrode implant and sham stimulation. i–l, Comparison of male and female control mice (i, k) and mice with vMT activation (j, l) reveals no significant sex differences across any of the behaviours performed in response to the looming threat. Notably, both male and female mice tail-rattle in response to the looming threat. Data are mean ± s.e.m.; NS, not significant. See Supplementary Table 1 for statistical analysis and sample sizes.