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. 2021 Sep 14;12(1):335–345. doi: 10.1515/tnsci-2020-0177

Figure 2.

Figure 2

miR-485-5p improves pericyte viability and suppresses pericyte apoptosis of APP/PS1 mice. (a) The effect of 0, 2.5, 5, 10 mM Aβ40 on the viability of pericyte was detected using CCK-8 assay. (b) RT-qPCR analysis was performed to measure miR-485-5p level in pericyte treated with the above concentration of Aβ40. (c) RT-qPCR analysis was conducted to measure miR-485-5p level in pericytes transfected with Aβ40, Aβ40 + NC mimics, or Aβ40 + miR-485-5p. (d) The viability of pericytes in the above four groups was determined by CCK-8. (e) The apoptosis of pericytes in the above groups was determined by flow cytometry. (f) Western blot analysis was conducted to evaluate the levels of proteins (Bax, Bcl-2, and cleaved caspase-3) associated with cell apoptosis. The apoptosis ratio was measured by the ratio of Bcl-2/Bax protein level. * p < 0.05, ** p < 0.01, NS: not significant.