KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-HIM-3 | Novus | Novus Cat# 53470002; RRID:AB_11013811 |
| Rabbit polyclonal anti-REC-8 | Novus | Novus Cat# 49230002; RRID:AB_10717927 |
| Mouse monoclonal anti-FLAG (M2) | Sigma | Sigma Cat# F3165; RRID:AB_259529 |
| Mouse monoclonal anti-RNA Polymerase II (8WG16) | BioLegend | BioLegend Cat# 664906; RRID:AB_2565554 |
| Rabbit polyclonal anti-GFP | Clontech | Takara Bio Cat# 632592; RRID:AB_2336883 |
| Goat anti-Mouse IgG (H+L) Alexa Fluor Plus 647 | Thermofisher Scientific | Invitrogen Cat# A32728; RRID:AB_2633277 |
| Goat anti-Rabbit IgG (H+L) Alexa Fluor Plus 488 | Thermofisher Scientific | Invitrogen Cat# A32731; RRID:AB_2633280 |
| Bacterial and virus strains | ||
| E. coli OP50 | CGC | OP50 |
| E. coli HT115 | CGC | HT115 |
| E. coli MG1693 | CGSC | MG1693 |
| Chemicals, peptides, and recombinant proteins | ||
| Indole-3-acetic acid (auxin) | Sigma | Cat# I2886-5G; Cas# 87-51-4 |
| IPTG | Thermofisher Scientific | Cat# R0393; Cas# 367-93-1 |
| Trizol reagent | Thermofisher Scientific | Cat #15596026 |
| Protein-G Dynabeads | Thermofisher Scientific | Cat # 10004D |
| EdU (5-ethynyl-2′-deoxyuridine) | Thermofisher Scientific | Cat # A10044 |
| Paraformaldehyde | Fisher Scientific | Cat# AA433689M; Cas# 30525-89-4 |
| iTaq Universal SYBR Green Supermix | BioRad | Cat# 1725124 |
| NEBuilderr HiFi DNA Assembly Master Mix | New England BioLabs | Cat# E2621 |
| Alt-R HiFi S.p. Cas9 NLS | IDT | Cat# 1078727 |
| Critical commercial assays | ||
| iScript cDNA synthesis kit | BioRad | Cat# 1708891 |
| Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye | Thermofisher Scientific | Cat# C10339 |
| Zymo Research Direct-zol RNA MiniPrep kit | Zymo Research | Cat # R2051 |
| ZymoPURE Plasmid Miniprep Kit | Zymo Research | Cat # R4208T |
| QIAGEN MinElute PCR Purification Kit | QIAGEN | Cat# 28004 |
| Deposited data | ||
| Tissue-specific HSF-1 occupancy at 20°C or upon heat shock (HS) at 34°C for 30 min (ChIP-seq) | This study | GEO: GSE162063 |
| Transcriptomic analyses of the heat shock response upon HSF-1 depletion from the somatic or germline tissue (RNA-seq) | This study | GEO: GSE162064 |
| Transcriptomic analyses of HSF-1 in germline development (RNA-seq) | This study | GEO: GSE162066 |
| Experimental models: Organisms/strains (C. elegans) | ||
| Wild type, Bristol | CGC | N2 |
| hsf-1(ok600) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III) | CGC | OG576 |
| unc-119(ed3)III;ieSi38[sun-1p::TIR1::mRuby::sun-1 3’UTR+Cbr-unc-119(+)]IV | CGC | CA1199 |
| ieSi57[eft-3p::TIR1::mRuby::unc-54 3’UTR+Cbr-unc-119(+)]II;unc-119(ed3)III | CGC | CA1200 |
| mkcSi13 II; rde-1(mkc36) V | CGC | DCL569 |
| daf-2(e1370)III | CGC | CB1370 |
| pdk-1(sa680)X | CGC | JT9609 |
| daf-16(mgDf50) I; daf-2(e1370) III | CGC | HT1890 |
| akt-1(ok525)V | CGC | RB759 |
| akt-2(ok393)X | CGC | VC204 |
| sqk-1(ok538)X | CGC | VC345 |
| hsf-1(ok600) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); ljtEx3[sur-5p::HSF-1 (cDNA)::gfp+ttx-3p::rfp] | This study | JTL804 |
| hsf-1(ljt3[hsf-1::degron::gfp])I; unc-119(ed3)III; ieSi38[sun-1p::TIR1 ::mRuby::sun-1 3′UTR+Cbr-unc-119(+)]IV | This study | JTL621 |
| hsf-1(ljt3[hsf-1::degron::gfp])I; ieSi57[eft-3p::TIR1:: mRuby::unc-54 3′ UTR+Cbr-unc-119(+)]II;unc-119(ed3)III | This study | JTL611 |
| daf-2(e1370) III; hsf-1(ljt4[3xFLAG::hsf-1::degron:: gfp])I; ieSi38[sun-1p::TIR1::mRuby::sun-1 3′UTR+Cbr-unc-119(+)]IV | This study | JTL708 |
| hsf-1(ok600) I; daf-2(e1370)III; ljtEx3[sur-5p::HSF-1 (cDNA)::gfp] | This study | JTL806 |
| hsf-1(ljt3[hsf-1::degron::gfp])I; daf-2(e1370)III; ieSi38[sun-1p::TIR1 ::mRuby::sun-1 3′UTR+Cbr-unc-119(+)]IV;rde-1(mkc36)V; ljtEx5[sur-5p::rde-1+ttx-3p::gfp+ myo-2p::rfp] | This study | JTL809 |
| Oligonucleotides | ||
| Please see Table S4 for a list of guide RNAs used in this study. | N/A | N/A |
| Please see Table S5 for a complete list of primers used in this study. | N/A | N/A |
| RNAi clones (multiple) | Ahringer Library; Bioscience | Cat# 3318_Cel_ RNAi_complete |
| Stellaris FISH Probes, Custom Assay with Quasar 670 Dye (multiple, please see Table S6 for sequences) | Biosearch Technologies | Cat# SMF-1065-5 |
| Recombinant DNA | ||
| pLZ29: pCFJ151_Peft-3_degron_EmGFP_unc-54 3′UTR | Zhang et al., 2015 | Addgene Plasmid #71719 |
| pSLGCV: a plasmid that contains sur-5p::luc+::gfp in pPD95.79 | Lagido et al., 2008 | Addgene Plasmid #49862 |
| pNP160: a plasmid that contains ttTi5605_SEC_ mtl-2p_RDE-1_3′rde-1 | a gift from Jonathan Ewbank, Aix-Marseille University | Addgene Plasmid #106362 |
| pPD97_75: empty backbone | a gift from Andrew Fire, Stanford University | Addgene Plasmid #1494 |
| sur-5p::HSF-1 (cDNA)::gfp in pPD95.79 | This study | N/A |
| sur-5p::rde-1 in pPD97_75 | This study | N/A |
| Software and algorithms | ||
| Imaris Image Analysis software | Bitplane | https://imaris.oxinst.com |
| Prism 8 | GraphPad Software | https://www.graphpad.com:443/ |
| Bowtie 2.3.5.1 | Langmead and Salzberg, 2012 | www.cs.jhu.edu/~langmea |
| Bedtools | Quinlan and Hall, 2010 | https://github.com/arq5x/bedtools2 |
| MACS 2.2.7.1 | Zhang et al., 2008 | https://github.com/macs3-project/MACS |
| RNA STAR 2.6 | Dobin et al., 2013 | https://github.com/alexdobin/STAR |
| Rsubread | Liao et al., 2019 | https://bioconductor.org/packages/release/bioc/html/Rsubread.html |
| edgR | Robinson et al., 2010 | http://bioconductor.org/packages/release/bioc/html/edgeR.html |
| IGV 2.5.0 | Robinson et al., 2011 | https://software.broadinstitute.org/software/igv/download |
| Ingenuity Pathway Analysis (IPA) | QIAGEN | https://digitalinsights.qiagen.com/products/ingenuity-pathway-analysis |