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. 2021 Sep 1;12:714750. doi: 10.3389/fmicb.2021.714750

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Copyright © 2021 Iantas, Savi, Schibelbein, Noriler, Assad, Dilarri, Ferreira, Rohr, Thorson, Shaaban and Glienke.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro assay to evaluate the activity of the extracts to inhibit the development of post-bloom fruit drop (PFD) in citrus flowers, 48 h after inoculation with 5 μL of conidial suspension of Colletotrichum abscissum (10⁶ conidia/mL; red arrows) added with: (a) 5 μL of saline solution (showing the characteristic symptom of PDF), (b) 5 μL of methanol (control), (c) 10 μL with carbendazim (Derosal – control), and 5 μL of each endophytic extracts: (d) Diaporthe vochysiae CMRP4322, (e) Nemania primolutea CMRP4323, (f) Pseudofusicoccum stromaticum CMRP4328, (g) D. vochysiae CMRP4321, (h) D. vochysiae CMRP4326, (i) D. vochysiae CMRP4332, (j) D. cerradensis CMRP4331, (k) D. cerradensis CMRP4324, (l) D. cf. heveae 1 CMRP4329, (m) Diaporthe sp. CMRP4330, (n) Coniochaeta sp. CMRP4325, and (o) Aspergillus sp. section Flavi CMRP4327.