FIGURE 5.

Expression levels of Nrf2 at 28 days after SCI in spinal cord tissue from WT and ApoE ^–/– mice (n = 6 samples per group). (a) Immunofluorescence staining of Nrf2 in WT mice. (b) Immunofluorescence staining of Nrf2 in ApoE ^–/– mice. Scale bar = 20 μm. Cell nuclei were stained with DAPI (blue). (c) Quantitative analysis of relative Nrf2 fluorescence intensity in experiments. (d) Western blot showing the expression of Nrf2 in the spinal cord from WT and ApoE ^–/– mice. β‐actin served as the internal control. (e) Quantitative analysis of relative Nrf2 protein expression in experiments in panel (d). All data are mean ± SD.

Abbreviations: ApoE, apolipoprotein E; DAPI, 4′,6‐diamidino‐2‐phenylindole; EA, electroacupuncture; Nrf2, nuclear factor erythroid 2‐related factor; SCI, spinal cord injury; WT, wild type. ^*** p < .001 vs. WT EA+COG112 group. ^### p < .001 vs. ApoE ^–/– EA+COG112 group