TABLE 2.
LMP1 interaction with RIP and TRAF3 in a yeast two-hybrid assaya
| Gal4 DBD-LMP1 (codons of LMP1) | Interaction with:
|
|
|---|---|---|
| Gal4 AD-RIP | Gal4 AD-TRAF3 | |
| 182–386 | + | ++ |
| 182–383ID | +/− | ++ |
| 182–386Δ185–211 | + | − |
| 182–386Δ212–240 | + | Not done |
| 187–231 | − | ++ |
| 355–386 | − | − |
a
S. cerevisiae Y190 was transformed with pAS1 vectors expressing the Gal4 DBD fused to the indicated codons of LMP1 and pACT vectors expressing the GAL4 AD fused to RIP codons 1 to 671 or TRAF3 codons 312 to 568. Transformed yeast cells were selected on medium lacking tryptophan and leucine. Colonies were transferred to filters and lysed by freeze thawing, and β-galactosidase was detected with X-Gal. Blue colonies after 1 h at 37°C were scored ++, and light blue colonies were scored +. Yeast cells transformed with these Gal4-DBD LMP1 DNAs alone scored negative for β-galactosidase.