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. Author manuscript; available in PMC: 2022 Aug 6.
Published in final edited form as: J Proteome Res. 2021 Jul 28;20(8):4031–4040. doi: 10.1021/acs.jproteome.1c00357

Figure 1. Schematic of the experimental workflow employed.

Figure 1.

Graphical representation of the workflow in which plasma is first depleted of high-abundance proteins, digested using either AspN, GluC, or Tryspin, and then individually separated by tip-based high-pH reverse phase fractionation. The resulting fractions are used to generate protease-specific sample libraries by DDA-PASEF(A). Lastly, un-depleted plasma samples digested with the same three proteases are analyzed using DIA-PASEF either individually, or as mixture (1:1:1) the three proteases for a give sample (MS pool). All DIA experiments utilize the aforementioned spectral library (B).