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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Biochem J. 2020 Feb 14;477(3):727–745. doi: 10.1042/BCJ20190874

Figure 5. In vitro and lysosomal stability of the TPP1 variants designed to improve hydrophobic packing of protein core.

Figure 5.

(A) Schematic of the methodology used to stabilize TPP1 via the M244F mutation. Structure as in Figure 2A with the M244 residue labeled in orange. The cavities within 6Å of M244 are labeled in magenta. . (B) Enzymatic half-life in vitro at 60°C. (C) Enzymatic half-life in the lysosome of LINCL lymphoblasts. ND = Not Determined