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. 2021 Aug 12;12(35):11882–11893. doi: 10.1039/d1sc02725e

Fig. 3. Darobactin A production was confirmed by uHPLC-HRMS analysis of the E. coli pNOSO-relEdarABCDE fermentation broth supernatant. Darobactin A is visualized as extracted ion chromatogram (EIC) at 483.71 Da (red trace) represents the [M + 2H]2+ MS peak, including the corresponding isotope pattern, which is shown in the grey field. The grey trace represents the base peak chromatogram (BPC) of the culture supernatant of E. coli BL21 (DE3) with and without the darobactin expression vector.

Fig. 3