Minimal inhibitory concentration (MIC in μg mL⁻¹) of darobactin 9 in an extended panel of clinically relevant Gram-negative pathogens. Darobactin A served as control. The heterologous darobactin producer strain E. coli BL21 (DE3) + BGC contains the pNOSO-darABCDE plasmid.

Strain		Dar. 9	Dar. A
E. coli	BL21 (DE3)	0.25	0.5–1
	BL21 (DE3) + BGC	2–4	8–16
	ATCC-25922	1–2	2
	JW0451-2 (ΔacrB)	0.125	0.5
	K12 ΔtolC	0.125	0.5
	ECO24a	1–2	8
	ECO25a	1–2	8
A. baumannii	DSM-30007	2	16
	DSM-30008	1–2	4
C. freundii	DSM-30039	0.5	1
E. cloacae	DSM-30054	1	2
K. pneumoniae	DSM-30104	1–2	2–4
	KPN12a	4	8
	KPN19a	1	2
	KPN62a	2	4
P. aeruginosa	PAO1	0.125	0.5–1
	PA14	2	16
	PA14 ΔmexAB	1	4
P. mirabilis	DSM-4479	32–64	64
P. vulgaris	DSM-2140	8	16
S. marcescens	DSM-30121	64	>64
E. faecalis	ATCC-29212	>64	>64
S. aureus	ATCC-29213	>64	>64

^aMDR clinical isolates (see ESI).