Comparison of H_AB determined at room temperature using the rates obtained from the RDE method and corresponding λ values with the help of Marcus equation at 113 mV overpotential.

Mutants	HAB (cm−1) η = 113 mV @ 298 K	λTotal (eV)
HtWT	0.84 ± 0.04	1.36 ± 0.03
HtQ64V	0.19 ± 0.01	1.20 ± 0.01
HtQ64N	0.31 ± 0.01	1.26 ± 0.02