Skip to main content
. 1999 Aug;19(8):5768–5784. doi: 10.1128/mcb.19.8.5768

TABLE 3.

Oligonucleotides

Oligonucleotide Sequence (5′-3′) Use
HL446 atgtgtaggttaagtgatcaaggcgtaatcaggcacatcatatggg
taggcgtaatcaggcacatcatatggg
tacatcaatacaaactcatcgacaactac
PCR with HA epitope
HL211 tgaaacatttgttttctttgggccggcaaacttattgtttttccaattg Bottom-strand oligonucleotide creates a unique NgoMI restriction site in gag in pHL1258
HL212 caattggaaaaacaataagtttgccggcccaaagaaaacaaatgtttca Top strand oligonucleotide creates a unit NgoMI restriction site in gag in pHL1258
HL220 gactacaaggacgacgatgacaag To generate a 24-bp DNA fragment encoding the FLAG epitope of pHL1276
HL221 cttgtcatcgtcgtccttgtagtc To generate a 24-bp DNA fragment encoding the FLAG epitope in pHL1276
HL199 gcaactagttcagatcttagtcgaccgatgcataaggatccctgagct 5′-flanking oligonucleotide of polylinker sequence in library vector pHL1288
HL200 cagggatccttatgcatcggtcgactaagatctgaactagttgctgca 3′-flanking oligonucleotide of polylinker sequence in library vector pHL1288
HL417 ctgcagcaactagttcagatcttagtcgaccgatgtataaggatccctgagctc 5′-flanking oligonucleotide in nup124 mutating ATG to a BclI site
HL340 gtcgatgagtttgtattgatcactcctgtttcaaaaaatac 3′-flanking fusion oligonucleotide for overlapping the pair HL417-HL418 with BclI mutation
HL418 ctagattcctaacctcgaggtataatcttgcttttgctttggc 3′-flanking oligonucleotide in nup124 mutating ATG to a BclI site
HL420 gtattttttgaaacaggagtgatcaatacaaactcatcgac 5′-flanking oligonucleotide for overlapping the pair HL417-HL418 with BclI mutation
HL447 catcgtaaggatccatttgtcttccaccaatttttgaatt PCR generation of the 3′ flank of S. pombe his3 gene with pHL1570 as template
HL440 gtatatacggccgtctatgcaaagctaacgaatct PCR generation of the 5′ flank of S. pombe his3 gene with pHL1570 as template
HL495 tttggcagatctctttcaacgttt PCR oligonucleotide 5′ flank for generation of Gag in GST vector
HL496 gctttaatcagaggatccatgaaaaactcatcacagaaaagaatt PCR oligonucleotide 3′ flank for generation of Gag in GST vector
HL520 agttagcacaaggataccctcgagtcaataacgtcttttcttgtattttgta PCR oligonucleotide 5′ flank for generation of an N-terminal portion of Nup124p in GST vector
HL510× catgctttaatcagaggatccagaattattcgcgttagtaatcggagccat PCR oligonucleotide 3′ flank for generation of an N-terminal portion of Nup124p in GST vector
HL521 agttagcacaaggataccgtcgactcacaaacgattagtttgcacatcagc PCR oligonucleotide 5′ flank for generation of C-terminal section of Nup124p in GST vector
HL501 catgctttaatcagaggatccaaggagaatgagcccaagccaacg PCR oligonucleotide 3′ flank for generation of C-terminal section of Nup124p in GST vector
HL339 gaattcatgcctcctgtttcaaaaaatac 5′ flank for 779–1073 nucleotide synthesis of nup124 segment 1 to generate LexA fusions
HL340 ctcgaggtataacttgcttttgctttgg 3′ flank for 779–1073 nucleotide synthesis of nup124 segment 1 to generate LexA fusions
HL341 gaattcagaattattcgcgttagtaatcgg 5′ flank for 1073–1718 nucleotide synthesis of nup124 segment 2 to generate LexA fusions
HL342 ctcgagtcatgcattgactaatacaggcttc 3′ flank for 1073–1718 nucleotide synthesis of nup124 segment 2 to generate LexA fusions
HL343 gaattcgaagttcagacagattccaacc 5′ flank for 1718–2363 nucleotide synthesis of nup124 segment 3 to generate LexA fusions
HL344 ctcgagtcacaaacgattagtttgcacaatca 3′ flank for 1718–2363 nucleotide synthesis of nup124 segment 3 to generate LexA fusions
HL345 gaattcaaggagaatgagcccaagcc 5′ flank for 2363–3008 nucleotide synthesis of nup124 segment 4 to generate LexA fusions
HL346 ctcgagtcacttggtaaaattaaatgaaaactg 3′ flank for 2363–3008 nucleotide synthesis of nup124 segment 4 to generate LexA fusions
HL347 gaattcccaaatacggatgccaaaacc 5′ flank for 3008–3653 nucleotide synthesis of nup124 segment 5 to generate LexA fusions
HL348 ctcgagtcaggtagtctttgaagttgcagac 3′ flank for 3008–3653 nucleotide synthesis of nup124 segment 5 to generate LexA fusions
HL349 gaattctctgaaggaaccgctccagc 5′ flank for 3653–4306 nucleotide synthesis of nup124 segment 6 to generate LexA fusions
HL350 ctcgagttaacgttttcttcgacttcgg 3′ flank 3653–4306 nucleotide synthesis of nup124 segment 6 to generate LexA fusions
HL414 gaattcatgaaaaaactcatcacagaaaaggattcgaatggaaatgg 5′ flank for Gag to generate B42 activation domain fusions
HL355 ctcgagtcaataacgtcttttcttgtattttg 3′ flank for Gag to generate B42 activation domain fusions
HL613 ggtattcaattcaatttgggctcttcta
actcacaaacaaatgcgcccccgggccg
taaaattgctgtgccccgaagtcgaa
gaaaacgttccggaggaggtggaggagagctcatgggtattcctaac
5′-flanking PCR oligonucleotide for generating nup124::pk1(3) GFP SV40 poly(A) ura4+ integration tag
HL614 taaaagcgtccgtatgtgtaaacaaaaatgtaataattatt
ccttagcaattaatttaaaggacaaagtctatctaaac
tatcatatgagaaagtctttgctgatatgcctt
3′-flanking PCR oligonucleotide for generating nup124::pk1(3) GFP SV40 poly(A) ura4+ integration tag