TABLE 3.

Oligonucleotides

Oligonucleotide	Sequence (5′-3′)	Use
HL446	atgtgtaggttaagtgatcaaggcgtaatcaggcacatcatatggg taggcgtaatcaggcacatcatatggg tacatcaatacaaactcatcgacaactac	PCR with HA epitope
HL211	tgaaacatttgttttctttgggccggcaaacttattgtttttccaattg	Bottom-strand oligonucleotide creates a unique NgoMI restriction site in gag in pHL1258
HL212	caattggaaaaacaataagtttgccggcccaaagaaaacaaatgtttca	Top strand oligonucleotide creates a unit NgoMI restriction site in gag in pHL1258
HL220	gactacaaggacgacgatgacaag	To generate a 24-bp DNA fragment encoding the FLAG epitope of pHL1276
HL221	cttgtcatcgtcgtccttgtagtc	To generate a 24-bp DNA fragment encoding the FLAG epitope in pHL1276
HL199	gcaactagttcagatcttagtcgaccgatgcataaggatccctgagct	5′-flanking oligonucleotide of polylinker sequence in library vector pHL1288
HL200	cagggatccttatgcatcggtcgactaagatctgaactagttgctgca	3′-flanking oligonucleotide of polylinker sequence in library vector pHL1288
HL417	ctgcagcaactagttcagatcttagtcgaccgatgtataaggatccctgagctc	5′-flanking oligonucleotide in nup124 mutating ATG to a BclI site
HL340	gtcgatgagtttgtattgatcactcctgtttcaaaaaatac	3′-flanking fusion oligonucleotide for overlapping the pair HL417-HL418 with BclI mutation
HL418	ctagattcctaacctcgaggtataatcttgcttttgctttggc	3′-flanking oligonucleotide in nup124 mutating ATG to a BclI site
HL420	gtattttttgaaacaggagtgatcaatacaaactcatcgac	5′-flanking oligonucleotide for overlapping the pair HL417-HL418 with BclI mutation
HL447	catcgtaaggatccatttgtcttccaccaatttttgaatt	PCR generation of the 3′ flank of S. pombe his3 gene with pHL1570 as template
HL440	gtatatacggccgtctatgcaaagctaacgaatct	PCR generation of the 5′ flank of S. pombe his3 gene with pHL1570 as template
HL495	tttggcagatctctttcaacgttt	PCR oligonucleotide 5′ flank for generation of Gag in GST vector
HL496	gctttaatcagaggatccatgaaaaactcatcacagaaaagaatt	PCR oligonucleotide 3′ flank for generation of Gag in GST vector
HL520	agttagcacaaggataccctcgagtcaataacgtcttttcttgtattttgta	PCR oligonucleotide 5′ flank for generation of an N-terminal portion of Nup124p in GST vector
HL510×	catgctttaatcagaggatccagaattattcgcgttagtaatcggagccat	PCR oligonucleotide 3′ flank for generation of an N-terminal portion of Nup124p in GST vector
HL521	agttagcacaaggataccgtcgactcacaaacgattagtttgcacatcagc	PCR oligonucleotide 5′ flank for generation of C-terminal section of Nup124p in GST vector
HL501	catgctttaatcagaggatccaaggagaatgagcccaagccaacg	PCR oligonucleotide 3′ flank for generation of C-terminal section of Nup124p in GST vector
HL339	gaattcatgcctcctgtttcaaaaaatac	5′ flank for 779–1073 nucleotide synthesis of nup124 segment 1 to generate LexA fusions
HL340	ctcgaggtataacttgcttttgctttgg	3′ flank for 779–1073 nucleotide synthesis of nup124 segment 1 to generate LexA fusions
HL341	gaattcagaattattcgcgttagtaatcgg	5′ flank for 1073–1718 nucleotide synthesis of nup124 segment 2 to generate LexA fusions
HL342	ctcgagtcatgcattgactaatacaggcttc	3′ flank for 1073–1718 nucleotide synthesis of nup124 segment 2 to generate LexA fusions
HL343	gaattcgaagttcagacagattccaacc	5′ flank for 1718–2363 nucleotide synthesis of nup124 segment 3 to generate LexA fusions
HL344	ctcgagtcacaaacgattagtttgcacaatca	3′ flank for 1718–2363 nucleotide synthesis of nup124 segment 3 to generate LexA fusions
HL345	gaattcaaggagaatgagcccaagcc	5′ flank for 2363–3008 nucleotide synthesis of nup124 segment 4 to generate LexA fusions
HL346	ctcgagtcacttggtaaaattaaatgaaaactg	3′ flank for 2363–3008 nucleotide synthesis of nup124 segment 4 to generate LexA fusions
HL347	gaattcccaaatacggatgccaaaacc	5′ flank for 3008–3653 nucleotide synthesis of nup124 segment 5 to generate LexA fusions
HL348	ctcgagtcaggtagtctttgaagttgcagac	3′ flank for 3008–3653 nucleotide synthesis of nup124 segment 5 to generate LexA fusions
HL349	gaattctctgaaggaaccgctccagc	5′ flank for 3653–4306 nucleotide synthesis of nup124 segment 6 to generate LexA fusions
HL350	ctcgagttaacgttttcttcgacttcgg	3′ flank 3653–4306 nucleotide synthesis of nup124 segment 6 to generate LexA fusions
HL414	gaattcatgaaaaaactcatcacagaaaaggattcgaatggaaatgg	5′ flank for Gag to generate B42 activation domain fusions
HL355	ctcgagtcaataacgtcttttcttgtattttg	3′ flank for Gag to generate B42 activation domain fusions
HL613	ggtattcaattcaatttgggctcttcta actcacaaacaaatgcgcccccgggccg taaaattgctgtgccccgaagtcgaa gaaaacgttccggaggaggtggaggagagctcatgggtattcctaac	5′-flanking PCR oligonucleotide for generating nup124::pk1(3) GFP SV40 poly(A) ura4+ integration tag
HL614	taaaagcgtccgtatgtgtaaacaaaaatgtaataattatt ccttagcaattaatttaaaggacaaagtctatctaaac tatcatatgagaaagtctttgctgatatgcctt	3′-flanking PCR oligonucleotide for generating nup124::pk1(3) GFP SV40 poly(A) ura4+ integration tag