TABLE 4.
Localization of transport substrates
| Transport substrate | Genotype of strain | Transport function studied | No. of nuclei observed
|
|
|---|---|---|---|---|
| Counteda | % Exhibiting nuclear localization | |||
| FLAG-tagged Gag | nup124+ | Import | 99a | 74 |
| FLAG-tagged Gag | nup124-1 | Import | 79a | 10 |
| SV40-NLS-GFP-LacZ | nup124+ | Import | 122b | 99 |
| SV40-NLS (Mut)-GFP-LacZ | nup124+ | Import | 66b | 0 |
| SV40-NLS-GFP-LacZ | nup124-1 | Import | 117b | 96 |
| SV40-NLS (Mut)-GFP-LacZ | nup124-1 | Import | 54b | 0 |
| Nucleoplasmin-NLS-GFP | nup124+ | Import | 88b | 98 |
| Nucleoplasmin-NLS (Mut)-GFP | nup124+ | Import | 89b | 0 |
| Nucleoplasmin-NLS-GFP | nup124-1 | Import | 89b | 98 |
| Nucleoplasmin-NLS (Mut)-GFP | nup124-1 | Import | 90b | 0 |
| Poly(A) mRNA | nup124+ | Export | 123c | 0.81 |
| Poly(A) mRNA | nup124-1 | Export | 153c | 1.96 |
| Poly(A) mRNA | rae1-1 | Export | 87c | 94 |
a
Only nuclei of cells displaying a Gag-specific FITC generated immunofluorescence (a), GFP-specific fluorescence (b), or FITC-conjugated anti-deoxygenin-generated immunofluorescence (c) were tabulated.