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. 2021 Sep 11:1–9. doi: 10.1080/14787210.2021.1976144

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

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Figure 1. — Checkerboard analysis for the development of the assay method. Each bar represents OD values. (a)The higher bar represents higher detection cross-reaction among test components. (b) Different concentrations of recombinant nucleocapsid (NCP) antigen challenge in various tests conditioned to determine the limit of detection. (c) Positive and negative sample challenge test to validate the method. Upon observing the cross-reaction values, the optimum condition was found with 1:100 dilution for monoclonal mouse anti-nucleocapsid antibody (MMAb), 1: 1000 dilution for monoclonal rabbit anti-nucleocapsid antibody (RMAb), and 1:4000 dilution for goat anti-rabbit secondary HRP conjugated antibody (G?R)