Fig. 1. INTS11 is required for fetal hematopoiesis.

(A) Relative mRNA levels of Ints11 in different hematopoietic cell populations from the BM of WT mice. n = 2 independent experiments; the hematopoietic populations were sorted from five WT mice in each experiment. Long-term HSC (LT-HSC), Lin⁻Sca1⁺cKit⁺CD34⁻CD135⁻; short-term HSC (ST-HSC), Lin⁻Sca1⁺cKit⁺CD34⁺CD135⁻; multipotent progenitor (MPP), Lin⁻Sca1⁺cKit⁺CD34⁺CD135⁺; common myeloid progenitor (CMP), LKS⁻CD34⁺CD16/32^intermediate; granulocyte-macrophage progenitor (GMP), LKS⁻CD34⁺CD16/32^high; megakaryocyte-erythroid progenitor (MEP), LKS⁻CD34⁻CD16/32^low. (B) Genotype distribution in the offspring of the intercross Vav1Cre⁺;Ints11^flox/+ × Vav1Cre⁻;Ints11^flox/flox mice on day 7 after birth. (C) Vav1Cre-mediated INTS11 depletion was verified in fetal liver CD45⁺ cells from E13.5 Vav1Cre⁺;Ints11^flox/flox embryos. (D and E) Representative embryos (D) and livers (E) from Vav1Cre⁺;Ints11^flox/flox and control littermates at E15.5. Photo credit: Peng Zhang, University of Texas Health Science Center at San Antonio. (F and G) Weight (F) and cellularity (G) of fetal livers from Vav1Cre⁺;Ints11^flox/flox (n = 10) and control littermates (n = 17) at E13.5. (H) Flow cytometric analysis of HSPCs (LSK and LKS⁻) from control and Vav1Cre⁺;Ints11^flox/flox fetal livers at E13.5. Lineage-negative cells are shown. (I and J) Quantification of the percentages of LKS⁻ (I) and LSK cells (J) from control (n = 11) and Vav1Cre⁺;Ints11^flox/flox (n = 7) fetal livers at E13.5. (K) CFU-C assay using E13.5 fetal liver cells from control (n = 7) and Vav1Cre⁺;Ints11^flox/flox embryos (n = 5) was assessed in semisolid medium in the presence of SCF, IL-3, TPO, GM-CSF, EPO, and IL-6. Data are means ± SEM. Unpaired Student’s t test: **P < 0.01 and ***P < 0.001.