Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 1;7(36):eabh1684. doi: 10.1126/sciadv.abh1684

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

PMC Copyright notice

Fig. 5. — (A) Venn diagram showing the overlap among binding sites (peaks) of INTS11 (GSE106359) and SUZ12 (GSE59090) in hematopoietic cells. (B) Gene ontology analysis of the genes that were co-occupied by INTS11 and SUZ12. (C) Representative ChIP-seq tracks show that INTS11 and SUZ12 co-occupy PRC2 target gene promoters in hematopoietic cells. (D) Nuclear extracts of 293T cells transfected with FLAG-tagged INTS11 or empty vector control (EV) were immunoprecipitated (IP) with anti-FLAG and probed for PRC2 proteins. Arrowhead indicates the FLAG-fusion protein. (E) Reciprocal SUZ12 IP from nuclear extracts of 293T cells transfected with FLAG-tagged INTS11 or EV, and representative immunoblot analysis. (F) Nuclear extracts of 293T cells transfected with HA-tagged EZH2 (top) or SUZ12 (bottom) were IP with anti-HA or mouse IgG, and Western blotting was performed. (G) Western blot analysis of the samples above (F) using antibodies against INT subunits INTS1, INTS3, and INTS4. (H) Soluble nuclear and chromatin-bound fractions from 293T cells transfected with HA-tagged SUZ12 were subjected to IP with anti-HA antibody followed by Western blot analysis with indicated antibodies.