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. 2021 Sep 8;7(37):eabj5363. doi: 10.1126/sciadv.abj5363

Fig. 3. Structure-functional analysis of SbmA.

Fig. 3.

(A) A cartoon representation of SbmA and glutamates, forming the glutamate ladder. (B) Transport assay data for the glutamate mutants. (C) A cartoon representation of SbmA showing Y116 (potential periplasmic gate) residue. (D) Antibiotic sensitivity of SbmA mutants upon conditions of low and high expression. Green: The mutation has no effect on transport, sensitive cells. Red: Cells become fully resistant at the level of ΔSbmA strain. Yellow: Resistant phenotype under low-expression conditions and low-level sensitivity under high-expression conditions (slow transport possible).