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. 2021 Sep 8;7(37):eabg4298. doi: 10.1126/sciadv.abg4298

Fig. 10. Externally supplied glutamate triggers calcium responses in distal leaves of Col-0 and msl10-1 mutants but not in glr3.3;3.6 double mutants.

Fig. 10.

(A) Calcium elevations triggered in L13 by application of 100 mM glutamate (Glu) to L8 at the indicated cut sites (white arrows). Col-0 and msl10-1 showed similar calcium elevations in response to Glu treatment, whereas glr3.3;3.6 double mutants showed no detectable response. (B) Averaged FI responses upon treatment with 100 mM Glu normalized to initial FI (FIt/FI0; n = 9 to 14). (C) Quantification and statistical analysis of maximal FI in L13. Violin plots and statistics as in Fig. 1 (n = 9 to 14). Corresponding data for mock-treated leaves are shown in fig. S14.