Table 1. Cryo-EM data collection, refinement, and validation statistics.
| E196K fibril (EMD-30887, PDB 7DWV) | |
| Data collection and processing | |
| Magnification | 29,000 |
| Voltage (kV) | 300 |
| Camera | K2 summit (Titan Krios) |
| Frame exposure time (s) | 0.2 |
| Movie frames (n) | 40 |
| Electron exposure (e−/Å2) | 64 |
| Defocus range (μm) | −3.0 to −1.5 |
| Pixel size (Å) | 1.014 |
| Symmetry imposed | C1 |
| Box size (pixel) | 400 |
| Interbox distance (Å) | 40.6 |
| Micrographs collected (n) | 4883 |
| Segments extracted (n) | 619,926 |
| Segments after Class2D (n) | 560,963 |
| Segments after Class3D (n) | 24,083 |
| Map resolution (Å) | 3.07 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 2.20–4.01 |
| Refinement | |
| Initial model used | 6LNI |
| Model resolution (Å) | 3.07 |
| FSC threshold | 0.143 |
| Model resolution range (Å) | 3.07 |
| Map sharpening B factor (Å2) | −43.26 |
| Model composition | |
| Nonhydrogen atoms | 2064 |
| Protein residues | 258 |
| Ligands | 0 |
| B factors (Å2) | |
| Protein | 101.56 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.007 |
| Bond angles (°) | 0.795 |
| Validation | |
| MolProbity score | 2.54 |
| Clashscore | 16.67 |
| Poor rotamers (%) | 0 |
| Ramachandran plot | |
| Favored (%) | 73.17 |
| Allowed (%) | 26.83 |
| Disallowed (%) | 0 |