FIG. 4.

Akt is sufficient to prevent alterations in mitochondrial dye uptake. (A) DiOC₆ labeling of mitochondria requires an intact inner membrane potential. (B) FACS analysis of UV-irradiated Rat1a and Rat1a/MyrAkt cells incubated with DiOC₆. Rat1a and Rat1a/MyrAkt cells were subjected to UV irradiation as described for Fig. 1, either mock treated or treated with 100 mM ClCCP for 5 min, and then loaded with DiOC₆ as described in Materials and Methods. Proliferating Rat1a cells loaded with DiOC₆ were defined as having a fluorescence of 500 arbitrary units.