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. 2021 Sep 8;7(37):eabi4852. doi: 10.1126/sciadv.abi4852

Fig. 2. Cancer-related anemia is caused by reduced RBC survival.

Fig. 2.

(A) RBC survival of Ctrl and TB mice was assessed by measuring biotinylated Ter119+ RBCs throughout the study. (B) As a measure for RBC eryptosis propensity and susceptibility for erythrophagocytosis, phosphatidylserine externalization in Ter119+ RBCs was assessed by annexin V binding. (C) Representative picture and spleen mass quantification of Ctrl and TB mice 3.5 weeks after tumor cell inoculation. The ruler is in centimeters. (D) Representative flow cytometry plots of viable splenocytes and relative and absolute quantification of CD45+ cells taking into account the spleen mass and cell number. (E) Spleen cell composition with regard to leukocytes was analyzed by flow cytometry. NK, natural killer; DC, dendritic cell. (F) CD45−CD71+ cells were assessed. Groups (n = 5 to 6 per group) were compared by two-tailed unpaired t tests with Welch’s correction and data are represented as means + SEM. Asterisks indicate differences between Ctrl and TB group. *P < 0.05, **P < 0.01, and ***P < 0.001. Photo credit: Regula Furrer, University of Basel.