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Kinetic parameters of the FNR as obtained for the diaphorase activity
| Enzyme | NADPH | |
|---|---|---|
| Km (μM) | kcat (s−1) | |
| EcFPR | 5 ± 1 | 49 ± 2 |
| PcFPR | 9 ± 1 | 50 ± 1 |
| BaFPR | 13 ± 1 | 146 ± 3 |
| LepFNR | 22 ± 1 | 280 ± 4 |
| PeaFNR | 29 ± 5 | 349 ± 20 |
Note: NADPH‐ferricyanide diaphorase activity in 50 mM Tris–HCl, pH 8.0, at 25°C. Values are mean ± SD of at least three independent measurements.
