TABLE 3.

Kinetic parameters of wild type and mutant EcFPR and PeaFNR as obtained for the diaphorase activity

Enzyme	NADPH
	Km (μM)	kcat (s−1)	Catalytic efficiency (μM−1 s−1)
EcFPR	5 ± 1	49 ± 2	10 ± 4
EcFPR_R144P	88 ± 10	83 ± 3	0.9 ± 0.3
EcFPR_R184Y	480 ± 112	20 ± 1	0.04 ± 0.02
EcFPR_R144P_R184Y	1289 ± 210	4.9 ± 0.3	0.004 ± 0.001
PeaFNR	29 ± 5	349 ± 20	12 ± 6
PeaFNR_P199R	28 ± 5	381 ± 20	14 ± 6
PeaFNR_Y240R	33 ± 4	283 ± 7	9 ± 3
PeaFNR_P199_Y240R	24 ± 1	225 ± 1	9 ± 2

Note: NADPH‐ferricyanide diaphorase activity in 50 mM Tris–HCl, pH 8.0, at 25°C. Values are mean ± SD of at least three independent measurements.