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. 2021 Sep 1;12:672252. doi: 10.3389/fphys.2021.672252

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Copyright © 2021 Parker, Tate, Prakoso, Deo, Willis, Nash, Donner, Crawford, Kiriazis, Granata, Coughlan, De Blasio and Ritchie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LV ROS production in diabetic mice. (A) LV Superoxide generation, (B) LV H₂O₂ generation, (C) LV superoxide/H₂O₂ ratio. Markers of LV ROS generation; gene expression of (D) superoxide dismutase-2 and (E) NADPH oxidase-4 (NOX4), and (F) protein abundance of NOX4. Data represented as mean ± SEM. n = 8–14 per group (note individual data points). Data analysis used unpaired t-test. *P < 0.05. ND, non-diabetic; D, diabetes.